To prove something works

1. Add inhibitor, see if it's turned off
2. Add activator, see if it's turned on, better yet, dose dependent
3. Over express the protein it self by insertion of expression vector, see again if it's turned on.
*. If possible, repeat in other cells/organism/protein analogue, see if results are situation specific or general.


Of all above, see if results are repeatable, and check if statistically significant.