Wednesday, January 14, 2009

Transformation in 10 minutes

This is totally amazing.

The Invitrogen Oneshot Top10 competent cells had 2 protocols on the instruction sheet. One is the traditional add DNA->on ice 30mins->heat shock->recover 1hr method, fair enough, although it's always a little annoying to wait for that 2 hours. But then the other method was a so-called "rapid" protocol:
Add DNA to cells -> on ice 5mins -> heat shock -> on ice 2 mins -> plate on a pre-warmed Agar plate.
And it worked wonders! Just in less then 10 minutes!

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